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NUTRIENT AGAR
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
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Medium for Escherichia coli DSM 499
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 1 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , for solid medium | 15 | g | 15 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Metadata
| Last modified: | 20.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Taxonomic range: | Phages |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2324 |
| Strains with modifications: | 183 |
Escherichia coli DSM 499
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | - |
| Peptone | 5 | - |
| Meat extract | 3 | - |
| Distilled water | - | - |
Cultivation metadata from 2324 strains
| halophily | non-halophilic | 7 strains |
| halophily | halotolerant | 1 strain |
| pH | 6 - 8 | 150 strains |
| pH | 4 - 6 | 95 strains |
| pH | 8 - 10 | 85 strains |
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