Strain modifications for Medium 1

Adjust pH of final medium to 7.0.

Adjust pH of final medium to 6.8 - 7.0.

2x concentrated

Add 1% glucose

Add 1% methanol

Add 1% methanol

Add 3% NaCl

Addition of vitamins for example see DSMZ medium 461) enhances reactivation and growth

develops better in liquid medium

Diluted 1:10, plus 1 g/l glucose

Grows on agar plates better than in liquid medium

Growth on solid medium

Incubate under 10% (v/v) O₂, 90% N₂

Liquid

Liquid medium for reactivation of freeze-dried culture

Liquid, with 20 % horse serum

medium with additionally 6% NaCl

No growth, only for rehydration of freeze-dryed material

One fifth diluted

Only liquid

Optimum 28°C

Plus 0,5 - 1 % methanol

Plus 0.1% Na-acetate

Plus 0.1% urea

Plus 0.2% urea

Plus 0.2% yeast extract

Plus 0.5% NaCl

Plus 1% glucose

Plus 1% MeOH

Plus 1% methanol

Plus 10 g/l NaCl

Plus 10 mg/l MnSO₄

Plus 10 mg/L MnSO₄; pH 6.0 - 8.0

Plus 10 mg/L MnSO₄; pH 7.0

Plus 10 mg/L MnSO₄; pH 8.0

Plus 10 µg/ml tetracycline

Plus 10% NaCl

Plus 10% NaCl; pH 7.5

Plus 10mg/l MnSO₄

Plus 20 mg/l uracil

Plus 200 mg/l NiCl₂

Plus 3% NaCl

Plus 4 g NaCl/L

Plus 5 g/l methanol; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 plus 5 g/l methanol. Subsequent subculturing may be carried out in liquid medium or on agar plates

Plus 5 g/l NaCl

Plus 5 g/l NaCl; Reactivate and grow freeze-dried cells from the ampoule in liquid medium 1 or 365. Subsequent subculturing may be carried out in liquid medium or on agar.

Plus 50% soil extract (see medium 80 or 12 for preparation)

Plus 500 µg/ml streptomycin

plus 0.1% Na₂CO₃

plus 0.1% urea

Plus 0.5% NaCl

Plus 1% MeOH

plus 1% methanol

plus 10 mg/L MnSO₄

plus 10 µg/ml MnSO₄

plus 100 g/L NaCl

plus 2% NaCl

Plus 4% NaCl

Plus 5 g/l NaCl

Plus 5 g/l NaCl plus 1 % (v/v) MeOH

plus 50 µg/ml thiamine

Plus 50% soil extract

Plus 50% soil extract and 5% NaCl

plus Manganese sulfate (10 µg/ml)

Psyhrotrophic, cultivate below 25 °C

Rehydrate and grow cells from the ampoule in liquid medium. Subsequent subculturing may be carried out in liquid medium or on agar plates

Rehydrate and grow freeze dried cells of ampoule in liquid medium 1. Subsequent subculturing may be carried out in liquid media or on agar plates.

Rehydrate and grow freeze-dried material from the ampoule in liquid medium 1. Subsequent subculturing may be carried out on medium 1 agar plates or in mineral medium with salicylate.

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 or 535. Subsequent subculturing may be carried out in liquid medium or on agar plates

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth medium 1. Subsequent subculturing may be carried out in liquid medium or on agar plates

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth medium 1. Subsequent subculturing may be carried out in liquid or agar medium 1 or on agar plates of medium 585

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 1. Subsequent subculturing may be carried out in liquid medium or on agar plates, in medium 1 or on.

Rehydrate and grow lyophilized cells from the ampoule in liquid broth 1 or 54. Subsequently, subculturing may be carried out in liquid medium or on agar plates

Rehydrate and grow lyophilized cells from the ampoule in liquid medium 1 or 381. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Rehydrate and grow lyophylisate in liquid medium 1. Subsequently, subculturing may be carried out in liquid medium or on agar plates

To reactivate freeze-dried cells, incubate them in liquid medium for one day before inoculating agar media.

With 0.2% glucose

With 0.2% pullulan

With 0.4% yeast extract and 0.2% benzoate

With 0.4% yeast extract and 0.2% sodiumbenzoate

With 0.5% NaCl

With 0.8 g/l NaCl

With 1 % (v/v) methanol

With 1 mg vitamin B₁₂ (cyanocobalamine)

With 1% glycerol and 0.4% K- or Na-oxalate

With 1% glycerol and 0.4% K- or Na-oxalate; rehydrate and grow lyophylisate in liquid medium 1. Subsequently, subculturing may be carried out in liquid medium or on agar plates

With 1% v/v methanol

With 10 mg/L manganese sulfate

With 10 mg/L MnSO₄

With 25 µg/ml tetracycline

With 4 g/l yeast

With 4 g/l yeast extract

With 40 µg/ml Thymin

With 5 g/l NaCl

With 5 g/l NaCl; rehydrate freeze dried cells in 5 ml medium 545. Incubate this culture until growth occurs. Subsequently, cultivation may be carried out in medium 1 or medium 545 and on agar plates.

With 50 mg/l streptomycin sulfate

With 50% soil extract

With 50% soil extract and 0.3% yeast extract

With 80 IU Penicillin G

Plus 10% NaCl