302
302 .
NUTRIENT BROTH WITH 10% HORSE SERUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 302 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , if necessary | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 To autoclaved medium add: | |||||
|
Horse serum
(sterile) |
100 | ml | - | - | |
| 2 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Metadata
| Last modified: | 08.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Peptone | 5 | - |
| Meat extract | 3 | - |
| Horse serum | - | - |
| Distilled water | - | - |
