# 1: NUTRIENT AGAR


## Main sol. 1

5	g	Peptone	
3	g	Meat extract	
15	g	Agar, for solid medium	
1000	ml	Distilled water	

1. Adjust pH to 7.0.
2. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.