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DSMZ

DSMZ 429a . COLUMBIA BLOOD AGAR WITH CHARCOAL

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 429a #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000 ml - -
Activated charcoal 2 g 2 -
Horse blood 4 % 40 -
1 Add 2 g/l activated charcoal to the columbia agar base solution before autoclaving. Add 4% horse blood after cooling to 50°C. Swirl agar during suspending. To prevent dry agar surface, spread about 1 ml of sterile medium or water over the agar plates and briefly allow to soak into the agar before inoculation. Incubate Neisseria strains in a humid microaerobic atmosphere. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1.

Medium 1 #

1000
Solution occurs in 20 other media (first defined in Medium 1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , for solid medium 15 g 15 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Medium 220 #

1000
Solution occurs in 22 other media (first defined in Medium 220).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g - -
Soy peptone 5 g - -
NaCl 5 g - -
Agar 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).

Medium 215 #

1000
Solution occurs in 5 other media (first defined in Medium 215).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(Difco) 		37 g 37 -
Distilled water 1000 ml - -

Metadata

Last modified: 19.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Horse blood 40 -
Activated charcoal 2 -
Columbia agar base - -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain