Main sol. 429a
Internal Solution-ID: S869
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Columbia agar base | 1000 | ml | - | - | |
| Activated charcoal | 2 | g | 2 | - | |
| Horse blood | 4 | % | 40 | - | |
| 1 Add 2 g/l activated charcoal to the columbia agar base solution before autoclaving. Add 4% horse blood after cooling to 50°C. Swirl agar during suspending. To prevent dry agar surface, spread about 1 ml of sterile medium or water over the agar plates and briefly allow to soak into the agar before inoculation. Incubate Neisseria strains in a humid microaerobic atmosphere. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1. | |||||
Media with this solution
| 429a | COLUMBIA BLOOD AGAR WITH CHARCOAL |