Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Columbia agar base | 1000 | ml | ||
|
Activated charcoal | 2 | g | ||
|
Horse blood | 4 | % | ||
1 Add 2 g/l activated charcoal to the columbia agar base solution before autoclaving. Add 4% horse blood after cooling to 50°C. Swirl agar during suspending. To prevent dry agar surface, spread about 1 ml of sterile medium or water over the agar plates and briefly allow to soak into the agar before inoculation. Incubate Neisseria strains in a humid microaerobic atmosphere. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Peptone | 5 | g | ||
|
Meat extract | 3 | g | ||
|
Agar , for solid medium | 15 | g | ||
|
Distilled water | 1000 | ml | ||
1 Adjust pH to 7.0. | |||||
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Casein peptone | 15 | g | ||
|
Soy peptone | 5 | g | ||
|
NaCl | 5 | g | ||
|
Agar | 15 | g | ||
|
Distilled water | 1000 | ml | ||
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). |