429d
429d .
FRANCISELLA HALIOTICIDA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 429d #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Columbia agar base
(dissolved in 70 % artificial seawater) |
1000.0 | ml | 700 | - | |
| Agar | 2.0 | g/l | 2 | 5.947 | |
| Horse blood OR Sheep blood | 4.0 | % | 40 | - | |
| Glucose | 1.0 | % | 10 | 55.506 | |
| FeCl3 | 2.0 | mM | 0.324 | 2 | |
| Cysteine | 0.1 | % | 1 | 8.254 | |
| 1 Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50°C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. | |||||
Solution occurs in 22
other media (first defined in Medium 220).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 15 | g | - | - | |
| Soy peptone | 5 | g | - | - | |
| NaCl | 5 | g | - | - | |
| Agar | 15 | g | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). | |||||
Solution occurs in 5
other media (first defined in Medium 215).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Brain heart infusion
(Difco) |
37 | g | 37 | - |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 19.11.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Columbia agar base | 700 | - |
| Horse blood | 40 | - |
| Sheep blood | 40 | - |
| Glucose | 10 | 55.506 |
| Agar | 2 | 5.947 |
| Show more | ||
