687
687 .
COLUMBIA BLOOD AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 687 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Columbia Blood Agar Base | 44 | g | - | - | |
| Deionized water | 1000 | ml | - | - | |
|
Defibrinated Blood
(sterile) |
5 | % | 50 | - | |
| 1 Suspend 44 g Columbia Blood Agar Base in 1l deionized water. Heat to boiling to dissolve completely. | |||||
| 2 Sterilize in autoclave for 15 min at 121°C. | |||||
| 3 Cool to 45°C. Add 5% sterile defibrinated blood and mix well. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Bacto Panton
(Peptone) |
10 | g | 10 | - |
|
Bacto Bitone
(Peptone) |
10 | g | 10 | - |
| Tryptic Digest of beef heart | 3 | g | 3 | - |
| Corn starch | 1 | g | 1 | - |
| NaCl | 5 | g | 5 | 85.558 |
| Agar | 15 | g | 15 | 44.599 |
Metadata
| Last modified: | 12.01.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |