DSMZ 429 . COLUMBIA BLOOD AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Conchiformibius steedae DSM 2580

* Modification: Add enough liquid medium 220, 535 or 215 to the agar plate when inoculating. The surface of agar plates must be moist during the whole incubation period

The modifications for this strain were extracted automatically and have not been curated by experts, yet.

Growth conditions: 37 °C
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000 ml - -
Horse blood 4 % 40 -
1 Columbia agar base supplemented with 4% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion).
Solution occurs in 4 other media (first defined in Medium 54a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D(+)-Glucose 2.5 g 2.5 13.877
NaCl 5.0 g 5 85.558
K2HPO4 2.5 g 2.5 14.353
Distilled water 1000.0 ml - -
1 Adjust pH to 7.3.
2 The same as Caso Bouillon (Merck or Oxoid, Germany).
Solution occurs in 2 other media (first defined in Medium 429).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(Difco)
37 g 37 -
Distilled water 1000 ml - -
Solution occurs in 2 other media (first defined in Medium 429).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

Metadata

Last modified: 20.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 115
Strains with modifications: 43
Conchiformibius steedae DSM 2580

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Horse blood 40 -
Columbia agar base - -

Cultivation metadata from 115 strains BacDive

halophily halotolerant 1 strain
pH 6 - 8 4 strains
phyla 4 113 strains
nutrition chemoorganotroph 2 strains
oxygen microaerophile 44 strains
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