Name: REACTIVATION WITH LIQUID MEDIUM 545
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. 54a #

ml

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
54. GLUCOSE YEAST EXTRACT AGAR	1000	ml	-	-
545. TRYPTONE SOYA BROTH (TSB)	1000	ml	-	-
1 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 54 or 545. Subsequent subculturing may be carried out in liquid medium or on agar plates medium 54.

54. GLUCOSE YEAST EXTRACT AGAR #

1000

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Glucose	20	g	20	111.012
Yeast extract	10	g	10	-
CaCO₃ (light precipitate)	20	g	20	199.82
Agar , for solid medium	17	g	17	50.546
Distilled water	1000	ml	-	-

1000

Solution occurs in 4 other media (first defined in Medium 54a).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Casein peptone	17.0	g	17	-
Soy peptone	3.0	g	3	-
D(+)-Glucose	2.5	g	2.5	13.877
NaCl	5.0	g	5	85.558
K₂HPO₄	2.5	g	2.5	14.353
Distilled water	1000.0	ml	-	-
1 Adjust pH to 7.3.
2 The same as Caso Bouillon (Merck or Oxoid, Germany).

54	GLUCOSE YEAST EXTRACT MEDIUM
a	REACTIVATION WITH LIQUID MEDIUM 545
b	CLOSTRIDIUM SP. MEDIUM