54b
54b .
CLOSTRIDIUM SP. MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 54b #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 20 | g | 20 | 111.012 | |
| Yeast extract | 10 | g | 10 | - | |
|
CaCO3
(light precipitate) |
20 | g | 20 | 199.82 | |
|
Agar
, for solid medium
optional |
17 | g | 17 | 50.546 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Dissolve ingredients (note that most of the carbonate remains suspended) and sparge medium with 100% N2 gas for 30 -45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials vials while stirring to keep carbonate suspended and autoclave. | |||||
Metadata
| Last modified: | 31.01.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 8 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| CaCO3 | 20 | 199.82 |
| Glucose | 20 | 111.012 |
|
Agar optional |
17 | 50.546 |
| Yeast extract | 10 | - |
| Distilled water | - | - |
