Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
, for solid medium
|1 Dissolve ingredients (note that most of the carbonate remains suspended) and sparge medium with 100% N2 gas for 30 -45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials vials while stirring to keep carbonate suspended and autoclave.|
|Medium type:||Complex medium|
|Equipment needed:||Autoclave, Gassing station, Hungate tubes|
|Edit in Medium builder|
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|All strains for this medium:||8|
|Compound||Conc. [g/L]||Conc. [mM]|