DSMZ 54b . CLOSTRIDIUM SP. MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 20 g 20 111.012
Yeast extract 10 g 10 -
CaCO3
(light precipitate)
20 g 20 199.82
Agar , for solid medium
optional
17 g 17 50.546
Distilled water 1000 ml - -
1 Dissolve ingredients (note that most of the carbonate remains suspended) and sparge medium with 100% N2 gas for 30 -45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials vials while stirring to keep carbonate suspended and autoclave.

Metadata

Last modified: 31.01.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 9

Molecular composition

Compound Conc. [g/L] Conc. [mM]
CaCO3 20 199.82
Glucose 20 111.012
Agar
optional
17 50.546
Yeast extract 10 -
Distilled water - -

Cultivation metadata from 9 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 8 strains
oxygen anaerobe 7 strains
temperature mesophilic 8 strains
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