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DSMZ

DSMZ 977 . VULCANITHERMUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 977 #

ml
Equipment needed: Anaerobic workbench Hungate tubes Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.33 6.169
MgCl2 x 6 H2O 0.33 g 0.33 1.623
CaCl2 x 2 H2O 0.33 g 0.33 2.245
KCl 0.33 g 0.33 4.427
KNO3 0.33 g 0.33 3.264
NaCl 25.00 g 25 427.789
PIPES
(12mM) 		3.60 g 3.6 11.906
Yeast extract 0.50 g 0.5 -
Tryptone 1.00 g 1 14.069
Sucrose 1.00 g 1 2.921
Modified Wolin's mineral solution II 1.00 ml - -
Wolin's vitamin solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the medium anaerobically, under nitrogen, omitting the CaCl2, MgCl2, KNO3, tryptone, yeast extract, vitamins and sucrose. The pH should be 6.8. Dispense the medium into vessels suitable for anaerobic growth (Hungate tubes or serum bottles) under an atmosphere of nitrogen and autoclave. To the sterile, cooled medium add, from sterile stock solutions the CaCl2, MgCl2, KNO3, tryptone, yeast extract, vitamins and sucrose. The CaCl2, MgCl2, KNO3, tryptone, yeast extract, and sucrose stock solutions should be autoclaved, while the vitamin solution is sterile filtered. This strain grows anaerobically in the presence of nitrate, but aerobic growth has been reported provided the oxygen concentration does not exceed 16% (v/v). Under anaerobic conditions a redox agent, such as sulphide is not required.

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Medium 534 #

1000
Solution occurs in 1 other media (first defined in Medium 534).
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.65 g 0.65 8.719
NaBr 0.10 g 0.1 0.972
NH4Cl 0.15 g 0.15 2.804
K2HPO4 0.15 g 0.15 0.861
CaCl2 x 2 H2O 0.50 g 0.5 3.401
Trace element solution 10.00 ml - -
Distilled water 1000.00 ml - -
1 Sparge medium with 100% N2 gas mixture for 20 min to make it anoxic.
2 Adjust pH to 6.5-6.8 with 1N H2SO4.
3 Fill 10 ml of medium in serum bottles (100 ml) under a protective H2/CO2 atmosphere and tightly stopper.
4 Autoclave at 121 °C for 15 min.
5 after autoclaving add the following filter-sterilized solutions.
Solution A , 0.3 ml/10ml
(gassed with CO2) 		30.00 ml/l - -
Solution B , 0.3 ml/10ml
(gassed with N2) 		30.00 ml/l - -
6 apply xx bar H2/CO2 (80%/20%) + yy % O2 (Note: strain specific conditions need to be applied)
7 For more details on the medium see the original description (Huber et al. System. Appl. Microbiol. 15:340-351 (1992)).

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 534).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Modified Wolin's mineral solution II 1000 ml - -
1 To 1000.0 ml trace element solution in medium 141 add:
(NH4)2Ni(SO4)2 2 g 2 5.063
Na2WO4 10 mg 0.01 0.034
Na2SeO4 10 mg 0.01 0.053

Solution A #

30
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2 g 66.667 793.585
Distilled water 30 ml - -
1 prepared under CO2 in an ice-bath, filter-sterilise

Solution B #

30
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2 g 66.667 268.616
Distilled water 30 ml - -
1 Prepared under N2,autoclave 121°C, 15 min

Modified Wolin's mineral solution II #

1000
Solution occurs in 24 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
PIPES 3.6 11.906
Tryptone 1 14.069
Sucrose 1 2.921
Yeast extract 0.5 -
NH4Cl 0.33 6.169
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature thermophilic 1 strain