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Modified Wolin's mineral solution II
Internal Solution-ID: S5902
This solution is used in 24 media and was first defined in medium 700.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.000 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.500 | g | 0.5 | 2.958 | |
| NaCl | 1.000 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.180 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.020 | g | 0.02 | 0.042 | |
| H3BO3 | 0.010 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
| Na2SeO3 x 5 H2O | 0.300 | mg | 3.0e-4 | 0.001 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Media with this solution
| 534 | MODIFIED SME MEDIUM |
| 597 | SALINIVIBRIO COSTICOLA SUBSP. VALLISMORTIS MEDIUM |
| 700 | ALKALIPHILIC SPIROCHAETE MEDIUM |
| 701 | HALOANAEROBACTER CHITINOVORANS MEDIUM |
| 761 | HALOTHERMOTHRIX ORENII |
| 764 | HALOANAEROBIUM LACUSROSEUS MEDIUM |
| 766 | THERMOCOCCUS CHITINOPHAGUS MEDIUM |
| 783 | HYDROGENOTHERMUS HIRSCHII MEDIUM |
| 806 | THERMOCOCCUS MEDIUM |
| 887 | OS MEDIUM |
| 889 | ISS1 MEDIUM |
| 890 | VM1 MEDIUM |
| 891 | SME MEDIUM MODIFIED FOR IS7 |
| 926 | ALKALIPHILIC THERMOCOCCUS MEDIUM |
| 930 | NATRONINCOLA HISTIDINOVORANS MEDIUM |
| 932 | HALONATRONUM SACCHAROPHILUM MEDIUM |
| 933 | HALOANAEROBIUM CONGOLENSE MEDIUM |
| 973 | MARINITHERMUS HYDROTHERMALIS MEDIUM |
| 977 | VULCANITHERMUS MEDIUM |
| 979 | PETROBACTER SUCCINIMANDENS MEDIUM |
| 1131 | HYDROGENIVIRGA OKINAWENSIS MEDIUM |
| 1223 | TINDALLIA TEXCOCOENSIS MEDIUM |
| 1373 | IRD MEDIUM |
| 1573 | T 5 MEDIUM |