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DSMZ

DSMZ 700 . ALKALIPHILIC SPIROCHAETE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Main sol. 700 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 10.0 g 10 94.35
NaHCO3 15.0 g 15 178.557
NaCl 10.0 g 10 171.116
K2HPO4 0.2 g 0.2 1.148
NH4Cl 1.0 g 1 18.695
KCl 0.2 g 0.2 2.683
Na2S x 9 H2O 1.0 g 1 4.164
Yeast extract 0.5 g 0.5 -
Sucrose 5.0 g 5 14.607
Wolin's vitamin solution 10.0 ml - -
Modified Wolin's mineral solution II 1.0 ml - -
Distilled water 1000.0 ml - -
1 Prepare the medium under N2, omitting Na2CO3, Na HCO3, Na2S, sucrose and vitamin solution.
2 Boil the medium and cool under nitrogen, add the Na2CO3 and NaHCO3 and adjust the pH to 9.7 with 6N NaOH (about 15 ml). Sterilize by autoclaving and add the Na2S (neutralized), vitamins and sucrose from sterile stock solutions.

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Modified Wolin's mineral solution II #

1000
Solution occurs in 24 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.7
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 15 178.557
Na2CO3 10 94.35
Sucrose 5 14.607
NH4Cl 1 18.695
Na2S x 9 H2O 1 4.164
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 2 strains
temperature mesophilic 3 strains