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Main sol. 534
Internal Solution-ID: S1179
This solution is used in 2 media and was first defined in medium 534.
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 30 | 513.347 | |
| MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
| MgCl2 x 6 H2O | 5.50 | g | 5.5 | 27.053 | |
| KCl | 0.65 | g | 0.65 | 8.719 | |
| NaBr | 0.10 | g | 0.1 | 0.972 | |
| NH4Cl | 0.15 | g | 0.15 | 2.804 | |
| K2HPO4 | 0.15 | g | 0.15 | 0.861 | |
| CaCl2 x 2 H2O | 0.50 | g | 0.5 | 3.401 | |
| Trace element solution | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Sparge medium with 100% N2 gas mixture for 20 min to make it anoxic. | |||||
| 2 Adjust pH to 6.5-6.8 with 1N H2SO4. | |||||
| 3 Fill 10 ml of medium in serum bottles (100 ml) under a protective H2/CO2 atmosphere and tightly stopper. | |||||
| 4 Autoclave at 121 °C for 15 min. | |||||
| 5 after autoclaving add the following filter-sterilized solutions. | |||||
|
Solution A
, 0.3 ml/10ml
(gassed with CO2) |
30.00 | ml/l | - | - | |
|
Solution B
, 0.3 ml/10ml
(gassed with N2) |
30.00 | ml/l | - | - | |
| 6 apply xx bar H2/CO2 (80%/20%) + yy % O2 (Note: strain specific conditions need to be applied) | |||||
| 7 For more details on the medium see the original description (Huber et al. System. Appl. Microbiol. 15:340-351 (1992)). | |||||