MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 950 . ONR7a MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Marinobacter sp. DSM 26671

* Modification: May be grown with 1.0 % sodium pyruvate.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 25 °C

Main sol. 950 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 500 ml - -
Solution 2 450 ml - -
Solution 3 50 ml - -
Sodium pyruvate* 10 g/l - -
1 Autoclave the solutions separately. All solutions should be mixed after autoclaving when they have cooled to at least 50°C. For solid media add to the first solution 15 g/l of Bacto agar or 12 g/l of agarose (Sigma).

Solution 1 #

500
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 22.79 g 45.58 779.945
Na2SO4 3.98 g 7.96 56.041
KCl 0.72 g 1.44 19.316
NaBr 83.00 mg 0.166 1.613
NaHCO3 31.00 mg 0.062 0.738
H3BO3 27.00 mg 0.054 0.873
NaF 2.60 mg 0.005 0.124
NH4Cl 0.27 g 0.54 10.095
Na2HPO4 x 7 H2O 89.00 mg 0.178 1.254
TAPSO 1.30 g 2.6 10.028
Distilled water 500.00 ml - -
Agar , for solid medium, alternative 15.00 g 30 89.198
Agarose , for solid medium, alternative 12.00 g 24 38.065
1 adjust pH to 7.6 with NaOH

Solution 2 #

450
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 11.18 g 24.844 122.204
CaCl2 x 2 H2O 1.46 g 3.244 22.069
SrCl2 x 6 H2O 24.00 mg 0.053 0.2
Distilled water 450.00 ml - -

Solution 3 #

50
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 2 mg 0.04 0.201
Distilled water 50 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.6
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 8
Strains with modifications: 8
Marinobacter sp. DSM 26671

  10.0 g/l Sodium pyruvate

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
NaCl 22.79 389.973
Agar 15 44.599
Agarose 12 19.033
MgCl2 x 6 H2O 11.18 54.992
Sodium pyruvate 10 90.876
Show more
CSV
JSON

Cultivation metadata from 8 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 8 strains
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
Show more