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Solution 1
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Internal Solution-ID: S1963
Equipment needed:
Autoclave
Compound
Amount
Unit
Conc. [g/L]
Conc. [mM]
NaCl
22.79
g
45.58
779.945
Na
2
SO
4
3.98
g
7.96
56.041
KCl
0.72
g
1.44
19.316
NaBr
83.00
mg
0.166
1.613
NaHCO
3
31.00
mg
0.062
0.738
H
3
BO
3
27.00
mg
0.054
0.873
NaF
2.60
mg
0.005
0.124
NH
4
Cl
0.27
g
0.54
10.095
Na
2
HPO
4
x 7 H
2
O
89.00
mg
0.178
1.254
TAPSO
1.30
g
2.6
10.028
Distilled water
500.00
ml
-
-
Agar
,
for solid medium, alternative
15.00
g
30
89.198
Agarose
,
for solid medium, alternative
12.00
g
24
38.065
1
adjust pH to 7.6 with NaOH
Media with this solution
950
ONR7a MEDIUM
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