950 .
ONR7a MEDIUM
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* Medium for Marinobacter sp. DSM 26671
May be grown with 1.0 % sodium pyruvate.
Growth conditions: 25 °C
Equipment needed:
Autoclave
Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution 1 | 500 | ml | ||
|
|
Solution 2 | 450 | ml | ||
|
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Solution 3 | 50 | ml | ||
|
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Sodium pyruvate | 10 | g/l | ||
| 1 Autoclave the solutions separately. All solutions should be mixed after autoclaving when they have cooled to at least 50°C. For solid media add to the first solution 15 g/l of Bacto agar or 12 g/l of agarose (Sigma). | |||||
Solution 1 #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 22.79 | g | ||
|
|
Na2SO4 | 3.98 | g | ||
|
|
KCl | 0.72 | g | ||
|
|
NaBr | 83.00 | mg | ||
|
|
NaHCO3 | 31.00 | mg | ||
|
|
H3BO3 | 27.00 | mg | ||
|
|
NaF | 2.60 | mg | ||
|
|
NH4Cl | 0.27 | g | ||
|
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Na2HPO4 x 7 H2O | 89.00 | mg | ||
|
|
TAPSO | 1.30 | g | ||
|
|
Distilled water | 500.00 | ml | ||
|
|
Agar , for solid medium, alternative | 15.00 | g | ||
|
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Agarose , for solid medium, alternative | 12.00 | g | ||
| 1 adjust pH to 7.6 with NaOH | |||||
Solution 2 #
450
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgCl2 x 6 H2O | 11.18 | g |
|
|
CaCl2 x 2 H2O | 1.46 | g |
|
|
SrCl2 x 6 H2O | 24.00 | mg |
|
|
Distilled water | 450.00 | ml |