938
938 .
ST21 AGAR FOR CELLULOSE DEGRADERS
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 938 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 600 | ml | - | - | |
| Solution B | 300 | ml | - | - | |
| Solution C | 100 | ml | - | - | |
| 1 Autoclave solutions separately and combine after cooling to 50°C. A slight precipitate does not restrict agar quality. Adjust pH to 7.2 with KOH using pH indicating paper strips before pouring plates. Place 3-4 strips of filter paper (ca 1 cm2) on the gelated plates and inoculate the filter strips. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| K2HPO4 | 1 | g | 1.667 | 9.569 |
|
Yeast extract
(Difco) |
20 | mg | 0.033 | - |
| Agar | 14 | g | 23.333 | 69.376 |
| Tap water | 600 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KNO3 | 1.0 | g | 3.333 | 32.97 |
| MgSO4 x 7 H2O | 1.0 | g | 3.333 | 13.524 |
| CaCl2 x 2 H2O | 1.0 | g | 3.333 | 22.673 |
| MnSO4 x 7 H2O | 0.1 | g | 0.333 | 2.207 |
| Tap water | 300.0 | ml | - | - |
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeCl3 | 0.2 | g | 2 | 12.331 |
| Tap water OR Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 7 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 14 | 41.626 |
| K2HPO4 | 1 | 5.741 |
| KNO3 | 1 | 9.891 |
| CaCl2 x 2 H2O | 1 | 6.802 |
| MgSO4 x 7 H2O | 1 | 4.057 |
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