938 .
ST21 AGAR FOR CELLULOSE DEGRADERS
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Equipment needed:
Autoclave
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 600 | ml | ||
|
|
Solution B | 300 | ml | ||
|
|
Solution C | 100 | ml | ||
| 1 Autoclave solutions separately and combine after cooling to 50°C. A slight precipitate does not restrict agar quality. Adjust pH to 7.2 with KOH using pH indicating paper strips before pouring plates. Place 3-4 strips of filter paper (ca 1 cm2) on the gelated plates and inoculate the filter strips. | |||||
Solution A #
600
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
K2HPO4 | 1 | g |
|
|
Yeast extract
(Difco) |
20 | mg |
|
|
Agar | 14 | g |
|
|
Tap water | 600 | ml |
Solution B #
300
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KNO3 | 1.0 | g |
|
|
MgSO4 x 7 H2O | 1.0 | g |
|
|
CaCl2 x 2 H2O | 1.0 | g |
|
|
MnSO4 x 7 H2O | 0.1 | g |
|
|
Tap water | 300.0 | ml |