925
925 .
ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thioalkalispira microaerophila DSM 14786
* Modification: Thioalkalispira microaerophila, ALEN 1 = DSM 14786. The strain is grown under nitrogen with 1% oxygen in the gas phase. The strain grows slowly with a potential lag phase of several days. Use 30 mM thiosulphate and up to a maximum of 5 mM NH4Cl
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C
Main sol. 925 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 20 | g | 20 | 188.701 | |
| NaHCO3 | 10 | g | 10 | 119.038 | |
| NaCl | 5 | g | 5 | 85.558 | |
| K2HPO4 | 1 | g | 1 | 5.741 | |
| Trace element solution | 2 | ml/l | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10. | |||||
| 2 After sterilization add: | |||||
|
MgCl2 x 6 H2O
(200.0 g/l) |
1 | ml/l | 0.2 | 0.984 | |
| 3 (a white colloid will form which will rapidly dissolve after mixing) | |||||
| Sodium thiosulphate* | 30 | mM | - | - | |
| NH4Cl* | 5 | mM | - | - | |
Solution occurs in 5
other media (first defined in Medium 925).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5 | mg | 0.005 | 0.017 | |
| FeSO4 x 7 H2O | 2 | mg | 0.002 | 0.007 | |
| ZnSO4 x 7 H2O | 100 | mg | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 30 | mg | 0.03 | 0.152 | |
| CoCl2 x 6 H2O | 200 | mg | 0.2 | 1.54 | |
| NiCl2 x 6 H2O | 20 | mg | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 30 | mg | 0.03 | 0.124 | |
| CuCl2 x 2 H2O | 10 | mg | 0.01 | 0.059 | |
| H3BO3 | 300 | mg | 0.3 | 4.851 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 10.0 |
| Edit in Medium builder | |
Gas composition
- 1 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2CO3 | 20 | 188.701 |
| NaHCO3 | 10 | 119.038 |
| NaCl | 5 | 85.558 |
| K2HPO4 | 1 | 5.741 |
| MgCl2 x 6 H2O | 0.2 | 0.984 |
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