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Trace element solution

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Internal Solution-ID: S1904

This solution is used in 6 media and was first defined in medium 925.

Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5 mg 0.005 0.017
FeSO4 x 7 H2O 2 mg 0.002 0.007
ZnSO4 x 7 H2O 100 mg 0.1 0.348
MnCl2 x 4 H2O 30 mg 0.03 0.152
CoCl2 x 6 H2O 200 mg 0.2 1.54
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 30 mg 0.03 0.124
CuCl2 x 2 H2O 10 mg 0.01 0.059
H3BO3 300 mg 0.3 4.851
Distilled water 1000 ml - -
1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.
925 ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM
1225 THIOALKALIBACTER HALOPHILUS MEDIUM
1228 THIOALKALIVIBRIO THIOCYANODENITRIFICANS MEDIUM
1229 THIOHALOSPIRA ALKALIPHILA MEDIUM
1230 THIOALKALIMICROBIUM MICROAEOPHILUM MEDIUM
1272 THIOALKALIVIBRIO JANNASCHI