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DSMZ

DSMZ 1230 . THIOALKALIMICROBIUM MICROAEOPHILUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1230 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 6.0 g 6 102.669
K2HPO4 2.0 g 2 11.483
NaHCO3 40.0 g 40 476.151
KNO3 0.5 g 0.5 4.945
Distilled water 1000.0 ml - -
1 Adjust the pH to 9.0 - 9.5 with 2M KOH. Dispense into closed vessels (i.e. a serum tube or bottle) with a liquid to air ratio of about 1:10. Flush the gas phase with nitrogen and add 2% oxygen. Sterilize at 120°C 20 min.
2 After sterilization add from sterile stock solutions:
Trace element solution 1.0 ml - -
MgCl2 x 6 H2O
(200.0 g/l) 		1.0 ml - -
3 (a white colloid will form which will rapidly dissolve after mixing)
Sodium thiosulfate pentahydrate
(4 M - 992 g/l) 		5.0 ml 4.964 20
4 Incubate statically until growth occurs, then shake at 100 rpm.

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 925).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5 mg 0.005 0.017
FeSO4 x 7 H2O 2 mg 0.002 0.007
ZnSO4 x 7 H2O 100 mg 0.1 0.348
MnCl2 x 4 H2O 30 mg 0.03 0.152
CoCl2 x 6 H2O 200 mg 0.2 1.54
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 30 mg 0.03 0.124
CuCl2 x 2 H2O 10 mg 0.01 0.059
H3BO3 300 mg 0.3 4.851
Distilled water 1000 ml - -
1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 9.0 - 9.5
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 40 476.151
NaCl 6 102.669
Sodium thiosulfate pentahydrate 4.964 20
K2HPO4 2 11.483
KNO3 0.5 4.945
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Cultivation metadata from 1 strains BacDive

pH 8 - 10 1 strain
phyla 1 1 strain
nutrition chemolithoautotroph 1 strain
oxygen microaerophile 1 strain
oxygen obligate aerobe 1 strain
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