Main sol. 1230
Internal Solution-ID: S2449
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 6.0 | g | 6 | 102.669 | |
| K2HPO4 | 2.0 | g | 2 | 11.483 | |
| NaHCO3 | 40.0 | g | 40 | 476.151 | |
| KNO3 | 0.5 | g | 0.5 | 4.945 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust the pH to 9.0 - 9.5 with 2M KOH. Dispense into closed vessels (i.e. a serum tube or bottle) with a liquid to air ratio of about 1:10. Flush the gas phase with nitrogen and add 2% oxygen. Sterilize at 120°C 20 min. | |||||
| 2 After sterilization add from sterile stock solutions: | |||||
| Trace element solution | 1.0 | ml | - | - | |
|
MgCl2 x 6 H2O
(200.0 g/l) |
1.0 | ml | - | - | |
| 3 (a white colloid will form which will rapidly dissolve after mixing) | |||||
|
Sodium thiosulfate pentahydrate
(4 M - 992 g/l) |
5.0 | ml | 4.964 | 20 | |
| 4 Incubate statically until growth occurs, then shake at 100 rpm. | |||||
Media with this solution
| 1230 | THIOALKALIMICROBIUM MICROAEOPHILUM MEDIUM |