{"medium":{"id":925,"name":"ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM","complex_medium":"no","min_pH":10,"max_pH":10,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium925.pdf"},"solutions":[{"id":1903,"name":"Main sol. 925","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na2CO3","compound_id":32,"amount":20,"unit":"g","g_l":20,"mmol_l":188.701,"optional":"no"},{"recipe No.":2,"compound":"NaHCO3","compound_id":55,"amount":10,"unit":"g","g_l":10,"mmol_l":119.038,"optional":"no"},{"recipe No.":3,"compound":"NaCl","compound_id":43,"amount":5,"unit":"g","g_l":5,"mmol_l":85.5578,"optional":"no"},{"recipe No.":4,"compound":"K2HPO4","compound_id":10,"amount":1,"unit":"g","g_l":1,"mmol_l":5.74132,"optional":"no"},{"recipe No.":5,"solution":"Trace element solution","solution_id":1904,"amount":2,"unit":"ml/l","optional":"no"},{"recipe No.":6,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"MgCl2 x 6 H2O","compound_id":79,"attribute":"200.0 g/l","amount":1,"unit":"ml/l","g_l":0.2,"mmol_l":0.983753,"optional":"no"}],"steps":[{"recipe No.":7,"step":"Sterilize at 110\u00b0C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10."},{"recipe No.":8,"step":"(a white colloid will form which will rapidly dissolve after mixing)"},{"recipe No.":7,"step":"After sterilization add:"}],"main solution":"yes"},{"id":1904,"name":"Trace element solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"EDTA","compound_id":271,"amount":5,"unit":"mg","g_l":0.005,"mmol_l":0.0171092,"optional":"no"},{"recipe No.":2,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.00719386,"optional":"no"},{"recipe No.":3,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.347752,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":30,"unit":"mg","g_l":0.03,"mmol_l":0.151588,"optional":"no"},{"recipe No.":5,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":200,"unit":"mg","g_l":0.2,"mmol_l":1.54037,"optional":"no"},{"recipe No.":6,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.154322,"optional":"no"},{"recipe No.":7,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":30,"unit":"mg","g_l":0.03,"mmol_l":0.123993,"optional":"no"},{"recipe No.":8,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.0586569,"optional":"no"},{"recipe No.":9,"compound":"H3BO3","compound_id":37,"amount":300,"unit":"mg","g_l":0.3,"mmol_l":4.85123,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Final pH should be 3, add HCl if needed. Sterilization - 120\u00b0C 20 min."}],"Sol. No.":49,"main solution":"no"}]}