Strain modifications for Medium 925

DSM 13531 = ARh1 (KSCN grown): Growth with thiocyanate : add KSCN up to 15 mM, incubation - 30C in conical flasks 1/10 liquid to air ratio, statically; growth is slow, about 1 week

DSM 13532 = ARh2 (KSCN grown): Growth with 15 mM KSCN: same conditions as for DSM 13531 = ARh1

DSM 13533 = ALRh; Strain ALRh: grows with 40 mM thiosulfate and 5 mM KSCN in conical flasks 1/5 liquid to air ratio at 200 rpm.

DSM 13541 = ARh1 (thiosulphate grown) Growth with thiosulfate (40 mM) and NH₄Cl (5 mM); in conical flasks 1/10 liquid/air ratio on shaker 200 rpm. Heavy sulfur formation is usual and it is necessary to continue cultivation until the sulfur has been utilized.

DSM 13542 = ARh2 (thiosulphate grown) Growth with thiosulfate (40 mM) and nitrate (10 mM); in conical flasks 1/5 liquid/air ratio on shaker 200 rpm. Growth takes 3-4 days, some sulfur can be formed during initial growth phase.

DSM 14477 = ALM1; Strain ALM1 grows with 40 mM thiosulphate and 5 mM KNO₃

Thioalkalimicrobium aerophilum AL 3 = DSM 13739 and Tm.sibericum AL 7 = DSM 13740 Use 80 mM thiosulphate, 5 mM KNO₃, reduce the amount of MgCl₂ to 0.5 mM

Thioalkalispira microaerophila, ALEN 1 = DSM 14786. The strain is grown under nitrogen with 1% oxygen in the gas phase. The strain grows slowly with a potential lag phase of several days. Use 30 mM thiosulphate and up to a maximum of 5 mM NH₄Cl

Thioalkalivibrio nitratireducens ALEN 2 = DSM 14787 Use 30 - 40 mM thiosulphate and 5-10 mM KNO₃

Thioalkalivibrio sp. ALED = DSM 14788 Use 30 mM thiosulphate and up to a maximum of 5 mM NH₄Cl

Thioalkalivibrio versutus AL 2 = DSM 13738, Tv.nitratus ALJ 12 = DSM 13741, and Tv.denitrificans ALJD = DSM 13742 Use 40 mM thiosulphate, 5 mM KNO₃, reduce the amount of MgCl₂ to 0.5 mM