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Main sol. 925
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Main sol. 925
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Internal Solution-ID: S1903
Compound
Amount
Unit
Conc. [g/L]
Conc. [mM]
Na
2
CO
3
20
g
20
188.701
NaHCO
3
10
g
10
119.038
NaCl
5
g
5
85.558
K
2
HPO
4
1
g
1
5.741
Trace element solution
2
ml/l
-
-
Distilled water
1000
ml
-
-
1
Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10.
2
After sterilization add:
MgCl
2
x 6 H
2
O
(200.0 g/l)
1
ml/l
0.2
0.984
3
(a white colloid will form which will rapidly dissolve after mixing)
Media with this solution
925
ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM
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