Main sol. 925
Internal Solution-ID: S1903
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 20 | g | 20 | 188.701 | |
| NaHCO3 | 10 | g | 10 | 119.038 | |
| NaCl | 5 | g | 5 | 85.558 | |
| K2HPO4 | 1 | g | 1 | 5.741 | |
| Trace element solution | 2 | ml/l | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10. | |||||
| 2 After sterilization add: | |||||
|
MgCl2 x 6 H2O
(200.0 g/l) |
1 | ml/l | 0.2 | 0.984 | |
| 3 (a white colloid will form which will rapidly dissolve after mixing) | |||||
Media with this solution
| 925 | ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM |