# 925: ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM


## Main sol. 925

20	g	Na2CO3	
10	g	NaHCO3	
5	g	NaCl	
1	g	K2HPO4	
2	ml/l	**Trace element solution**	
1000	ml	Distilled water	

1. Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10.
2. After sterilization add:

1	ml/l	MgCl2 x 6 H2O (200.0 g/l)	

3. (a white colloid will form which will rapidly dissolve after mixing)


## Trace element solution

5	mg	EDTA	
2	mg	FeSO4 x 7 H2O	
100	mg	ZnSO4 x 7 H2O	
30	mg	MnCl2 x 4 H2O	
200	mg	CoCl2 x 6 H2O	
20	mg	NiCl2 x 6 H2O	
30	mg	Na2MoO4 x 2 H2O	
10	mg	CuCl2 x 2 H2O	
300	mg	H3BO3	
1000	ml	Distilled water	

1. Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.