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DSMZ

DSMZ 925 . ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Thioalkalivibrio denitrificans DSM 13742

* Modification: Thioalkalivibrio versutus AL 2 = DSM 13738, Tv.nitratus ALJ 12 = DSM 13741, and Tv.denitrificans ALJD = DSM 13742 Use 40 mM thiosulphate, 5 mM KNO3, reduce the amount of MgCl2 to 0.5 mM

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C

Main sol. 925 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 20.0 g 20 188.701
NaHCO3 10.0 g 10 119.038
NaCl 5.0 g 5 85.558
K2HPO4 1.0 g 1 5.741
Trace element solution 2.0 ml/l - -
Distilled water 1000.0 ml - -
1 Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10.
2 After sterilization add:
MgCl2 x 6 H2O*
(200.0 g/l) 		0.5 mM - -
3 (a white colloid will form which will rapidly dissolve after mixing)
Sodium thiosulphate* 40.0 mM - -
KNO3* 5.0 mM - -

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 925).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5 mg 0.005 0.017
FeSO4 x 7 H2O 2 mg 0.002 0.007
ZnSO4 x 7 H2O 100 mg 0.1 0.348
MnCl2 x 4 H2O 30 mg 0.03 0.152
CoCl2 x 6 H2O 200 mg 0.2 1.54
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 30 mg 0.03 0.124
CuCl2 x 2 H2O 10 mg 0.01 0.059
H3BO3 300 mg 0.3 4.851
Distilled water 1000 ml - -
1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 10.0
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Associated strains

All strains for this medium: 13
Strains with modifications: 12
Thioalkalivibrio denitrificans DSM 13742

  40 mM Sodium thiosulphate
  5 mM KNO3
  0.5 mM MgCl2 x 6 H2O
  MgCl2 x 6 H2O

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 20 188.701
NaHCO3 10 119.038
NaCl 5 85.558
K2HPO4 1 5.741
MgCl2 x 6 H2O 0.2 0.984
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Cultivation metadata from 13 strains BacDive

pH 6 - 8 3 strains
pH 8 - 10 3 strains
pH 10 - 12 3 strains
phyla 1 13 strains
nutrition chemolithoautotroph 3 strains
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