830
830 .
R2A MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Novosphingobium stygium DSM 12445
* Modification: Rehydrate and grow freeze-dried cells from the ampoule in medium 830, subsequent subculturing may be carried out in medium 457 with carbon source or in medium 830 or 464.
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C
Main sol. 830 #
ml
Solution occurs in 9
other media (first defined in Medium 830).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.50 | g | - | - | |
|
Proteose peptone
(Difco no. 3) |
0.50 | g | - | - | |
| Casamino acids | 0.50 | g | - | - | |
| Glucose | 0.50 | g | - | - | |
|
Starch
(soluble) |
0.50 | g | - | - | |
| Na-pyruvate | 0.30 | g | - | - | |
| K2HPO4 | 0.30 | g | - | - | |
| MgSO4 x 7 H2O | 0.05 | g | - | - | |
| Agar | 15.00 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 0.5 | 2.775 |
| Casamino acids | 0.5 | - |
| Starch | 0.5 | - |
| Proteose peptone | 0.5 | - |
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Cultivation metadata from 1844 strains
| halophily | non-halophilic | 15 strains |
| halophily | moderately halophilic | 2 strains |
| pH | 6 - 8 | 489 strains |
| pH | 4 - 6 | 264 strains |
| pH | 8 - 10 | 242 strains |
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