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DSMZ

DSMZ 746 . RHODOVULUM STRICTUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 746 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 500 ml - -
Solution 2 500 ml - -
1 Boil both solutions under a stream of nitrogen gas for few minutes and autoclave separately under anaerobic conditions at 121C for 15 minutes. Mix aseptically after autoclaving under a stream of sterile nitrogen gas and adjust final pH to 7.8. Distribute in screw-capped bottles or tubes.

Solution 1 #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.00 g 2 15.136
NaCl 8.20 g 16.4 280.63
MgCl2 x 6 H2O 0.20 g 0.4 1.968
CaCl2 x 2 H2O 0.05 g 0.1 0.68
Trace element solution SL-8 1.00 ml - -
Na-DL-malate 3.60 g 7.2 40.438
Yeast extract 1.00 g 2 -
Na2S2O3 x 5 H2O 0.50 g 1 4.029
Distilled water 500.00 ml - -

Solution 2 #

500
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Potassium phosphate buffer
(pH 7.8, 20 mM) 		500 ml - 10
1 Boil both solutions under a stream of nitrogen gas for few minutes and autoclave separately under anaerobic conditions at 121°C for 15 minutes. Mix aseptically after autoclaving under a stream of sterile nitrogen gas and adjust final pH to 7.8. Distribute in screw-capped bottles or tubes.

Trace element solution SL-8 #

1000
Solution occurs in 2 other media (first defined in Medium 745).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 62.0 mg 0.062 1.003
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoSO4 x 2 H2O 36.0 mg 0.036 -
Distilled water 1000.0 ml - -
1 pH 6.8

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 8.2 140.315
Na-DL-malate 3.6 20.219
(NH4)2SO4 1 7.568
Yeast extract 1 -
Na2S2O3 x 5 H2O 0.5 2.015
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains