DSMZ 745 . RHODOBIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1 7.348
(NH4)2SO4 1.00 g 1 7.568
NaCl 50.20 g 50.2 859.001
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Trace element solution SL-8 1.00 ml - -
Na-DL-malate 3.60 g 3.6 20.219
Yeast extract 1.00 g 1 -
Na2S2O3 x 5 H2O 0.50 g 0.5 2.015
Distilled water 1000.00 ml - -
1 Boil the medium under a stream of nitrogen gas for few minutes and distribute in screw-capped bottles or tubes. Autoclave at 121°C for 15 minutes.
Solution occurs in 1 other media (first defined in Medium 745).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 62.0 mg 0.062 1.003
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoSO4 x 2 H2O 36.0 mg 0.036 -
Distilled water 1000.0 ml - -
1 pH 6.8

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 50.2 859.001
Na-DL-malate 3.6 20.219
(NH4)2SO4 1 7.568
KH2PO4 1 7.348
Yeast extract 1 -
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains