Solution 2
Internal Solution-ID: S1527
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Potassium phosphate buffer
(pH 7.8, 20 mM) |
500 | ml | - | 10 | |
| 1 Boil both solutions under a stream of nitrogen gas for few minutes and autoclave separately under anaerobic conditions at 121°C for 15 minutes. Mix aseptically after autoclaving under a stream of sterile nitrogen gas and adjust final pH to 7.8. Distribute in screw-capped bottles or tubes. | |||||
Media with this solution
| 746 | RHODOVULUM STRICTUM MEDIUM |