# 746: RHODOVULUM STRICTUM MEDIUM


## Main sol. 746

500	ml	**Solution 1**	
500	ml	**Solution 2**	

1. Boil both solutions under a stream of nitrogen gas for few minutes and autoclave
separately under anaerobic conditions at 121C for 15 minutes. Mix aseptically after
autoclaving under a stream of sterile nitrogen gas and adjust final pH to 7.8. Distribute
in screw-capped bottles or tubes.


## Solution 1

1	g	(NH4)2SO4	
8.2	g	NaCl	
0.2	g	MgCl2 x 6 H2O	
0.05	g	CaCl2 x 2 H2O	
1	ml	**Trace element solution SL-8**	
3.6	g	Na-DL-malate	
1	g	Yeast extract	
0.5	g	Na2S2O3 x 5 H2O	
500	ml	Distilled water	


## Solution 2

500	ml	Potassium phosphate buffer (pH 7.8, 20 mM)	

1. Boil both solutions under a stream of nitrogen gas for few minutes and autoclave separately under anaerobic conditions at 121°C for 15 minutes. Mix aseptically after autoclaving under a stream of sterile nitrogen gas and adjust final pH to 7.8. Distribute in screw-capped bottles or tubes. 


## Trace element solution SL-8

5.2	g	Na2-EDTA	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
62	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
17	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoSO4 x 2 H2O	
1000	ml	Distilled water	

1. pH 6.8