MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 573 . THIOTHRIX MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 573 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.20 g 0.2 3.739
K2HPO4 0.01 g 0.01 0.057
MgSO4 x 7 H2O 0.01 g 0.01 0.041
CaSO4
(saturated solution) 		20.00 ml - -
Trace element solution 5.00 ml - -
Na-acetate 0.10 g 0.1 1.219
Agar , if required 12.00 g 12 35.679
Distilled water 1000.00 ml - -
1 Adjust pH to 7.5 before autoclaving. Sterilize separately a 10% (w/v) solution of Na2S x 9 H2O; add the following amount of this solution to the medium after autoclaving (= shortly before using the medium).
neutralized Na2S x 9 H2O solution, 10% (w/v) 3.00 ml - -
2 Note: For small volumes, such as 5 mL agar slants, you can use the respective volume of a sterile neutralized 3% Na2S x 9 H2O solution (e.g., 50µL/5 mL = 0.3g/L)

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 155).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.20 g 0.2 0.684
FeSO4 x 7 H2O 0.70 g 0.7 2.518
ZnSO4 x 7 H2O 0.01 g 0.01 0.035
MnSO4 x 4 H2O 2.00 mg 0.002 0.009
CuSO4 x 5 H2O 5.00 µg 5.0e-6 2.0e-5
H3BO3 10.00 mg 0.01 0.162
Co(NO3)2 1.00 mg 1.0e-3 0.005
Na2MoO4 x 2 H2O 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -

neutralized Na2S x 9 H2O solution, 3% (w/v) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 3 %(w/v) 30 124.905
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

neutralized Na2S x 9 H2O solution, 10% (w/v) #

100
Solution occurs in 1 other media (first defined in Medium 573).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 10 g - -
Distilled water 100 ml - -
1 Adjust pH to 7.0.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Edit in Medium builder

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
Na2S x 9 H2O 0.3 1.249
NH4Cl 0.2 3.739
Na-acetate 0.1 1.219
K2HPO4 0.01 0.057
Show more
CSV
JSON

Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 4 strains
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
Show more