573 .
THIOTHRIX MEDIUM
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Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 0.20 | g | ||
|
|
K2HPO4 | 0.01 | g | ||
|
|
MgSO4 x 7 H2O | 0.01 | g | ||
|
|
CaSO4
(saturated solution) |
20.00 | ml | ||
|
|
Trace element solution | 5.00 | ml | ||
|
|
Na-acetate | 0.10 | g | ||
|
|
Agar , if required | 12.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.5 before autoclaving. Sterilize separately a 10% (w/v) solution of Na2S x 9 H2O; add the following amount of this solution to the medium after autoclaving (= shortly before using the medium). | |||||
|
|
neutralized Na2S x 9 H2O solution, 10% (w/v) | 3.00 | ml | ||
| 2 Note: For small volumes, such as 5 mL agar slants, you can use the respective volume of a sterile neutralized 3% Na2S x 9 H2O solution (e.g., 50µL/5 mL = 0.3g/L) | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
EDTA | 0.20 | g |
|
|
FeSO4 x 7 H2O | 0.70 | g |
|
|
ZnSO4 x 7 H2O | 0.01 | g |
|
|
MnSO4 x 4 H2O | 2.00 | mg |
|
|
CuSO4 x 5 H2O | 5.00 | µg |
|
|
H3BO3 | 10.00 | mg |
|
|
Co(NO3)2 | 1.00 | mg |
|
|
Na2MoO4 x 2 H2O | 1.00 | mg |
|
|
Distilled water | 1000.00 | ml |
neutralized Na2S x 9 H2O solution, 3% (w/v) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2S x 9 H2O | 3 | %(w/v) | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
neutralized Na2S x 9 H2O solution, 10% (w/v) #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2S x 9 H2O | 10 | g | ||
|
|
Distilled water | 100 | ml | ||
| 1 Adjust pH to 7.0. | |||||