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Main sol. 573

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Internal Solution-ID: S1236

Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.20 g 0.2 3.739
K2HPO4 0.01 g 0.01 0.057
MgSO4 x 7 H2O 0.01 g 0.01 0.041
CaSO4
(saturated solution) 		20.00 ml - -
Trace element solution 5.00 ml - -
Na-acetate 0.10 g 0.1 1.219
Agar , if required 12.00 g 12 35.679
Distilled water 1000.00 ml - -
1 Adjust pH to 7.5 before autoclaving. Sterilize separately a 10% (w/v) solution of Na2S x 9 H2O; add the following amount of this solution to the medium after autoclaving (= shortly before using the medium).
neutralized Na2S x 9 H2O solution, 10% (w/v) 3.00 ml - -
2 Note: For small volumes, such as 5 mL agar slants, you can use the respective volume of a sterile neutralized 3% Na2S x 9 H2O solution (e.g., 50µL/5 mL = 0.3g/L)
573 THIOTHRIX MEDIUM