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DSMZ

DSMZ 462a . PLATES WITH FLUORANTHENE

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 462a #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 462 1000 ml - -
Fluoranthene
(dissolved in acetone) 		trace 2 9.889
1 Pour plates of medium 462. Dissolve 2 mg/ml fluoranthene in acetone and spread 1 ml of this solution on the surface of every pre-dried plate. Allow the acetone to evaporate under sterile conditions. Inoculate as usual.

Medium 462 #

1000
Solution occurs in 1 other media (first defined in Medium 462).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 2.44 g 2.44 17.188
KH2PO4 1.52 g 1.52 11.17
(NH4)2SO4 0.50 g 0.5 3.784
MgSO4 x 7 H2O 0.20 g 0.2 0.811
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Trace element solution SL-4 10.00 ml - -
Vitamin solution
(Schlegel) 		2.50 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.9.
2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 462 with the appropriate carbon source.
Medium 1 1000.00 ml - -
Medium 220 1000.00 ml - -
Medium 535 1000.00 ml - -

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Vitamin solution #

100
Solution occurs in 4 other media (first defined in Medium 462).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoate 1.0 mg 0.01 0.073
Biotin 0.2 mg 0.002 0.008
Nicotinic acid 2.0 mg 0.02 0.162
Thiamine-HCl x 2 H2O 1.0 mg 0.01 0.027
Calcium pantothenate 0.5 mg 0.005 0.01
Pyridoxamine 5.0 mg 0.05 0.297
Vitamin B12 2.0 mg 0.02 0.015
Distilled water 100.0 ml - -

Medium 1 #

1000
Solution occurs in 20 other media (first defined in Medium 1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , for solid medium 15 g 15 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Medium 220 #

1000
Solution occurs in 22 other media (first defined in Medium 220).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g - -
Soy peptone 5 g - -
NaCl 5 g - -
Agar 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).

Medium 535 #

1000
Solution occurs in 18 other media (first defined in Medium 535).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459) 		30 g - -
Agar 20 g - -
Distilled water 1000 ml - -
1 pH 7.3
2 Autoclave at 121°C for 15 min.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 25.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 7
Strains with modifications: 5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 2.44 17.188
Fluoranthene 2 9.889
KH2PO4 1.52 11.17
(NH4)2SO4 0.5 3.784
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 7 strains BacDive

phyla 2 7 strains
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 7 strains