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JCM 340 . MBM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J340 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 0.2 g 0.2 2.348
KH2PO4 0.2 g 0.2 1.467
NH4Cl 0.2 g 0.2 3.732
MgCl2 x 6 H2O 0.4 g 0.399 1.964
KCl 0.2 g 0.2 2.677
CaCl2 x 2 H2O 0.1 g 0.1 0.679
Trace element solution SL-4 2.0 ml - -
Na2S x 9 H2O 0.5 g 0.499 2.078
Resazurin 1.0 mg 1.0e-3 0.004
Distilled water 1000.0 ml - -
1 Mix ingredients except SL-4 trace element solution and Na2S x 9H2O, and autoclave. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Filter-sterilize SL-4 trace element solution, and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a stream of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add the Na2S x 9H2O solution to the medium, and adjust pH to 7.0, if necessary.

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

SL-6 trace element solution #

1000
Solution occurs in 10 other media (first defined in Medium J167).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.499 2.078
MgCl2 x 6 H2O 0.399 1.964
NH4Cl 0.2 3.732
KCl 0.2 2.677
NaNO3 0.2 2.348
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain