Main sol. 462
Internal Solution-ID: S931
This solution is used in 2 media and was first defined in medium 462.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 | 2.44 | g | 2.44 | 17.188 | |
| KH2PO4 | 1.52 | g | 1.52 | 11.17 | |
| (NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| Trace element solution SL-4 | 10.00 | ml | - | - | |
|
Vitamin solution
(Schlegel) |
2.50 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 6.9. | |||||
| 2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 462 with the appropriate carbon source. | |||||
| Medium 1 | 1000.00 | ml | - | - | |
| Medium 220 | 1000.00 | ml | - | - | |
| Medium 535 | 1000.00 | ml | - | - | |