DSMZ 1a . REACTIVATION WITH LIQUID MEDIUM 1

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Culture medium recipe

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Medium for Comamonas testosteroni DSM 7300

* Modification: Rehydrate and grow freeze dried cells in liquid medium 1. Subsequent subcultivation may be carried out in liquid medium or on agar plates or in medium 457 plus sulfophthalate

The modifications for this strain were extracted automatically and have not been curated by experts, yet.

Growth conditions: 28 °C
1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , for solid medium 15 g 15 44.599
Distilled water 1000 ml - -
2 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
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Associated strains

All strains for this medium: 209
Strains with modifications: 9
Comamonas testosteroni DSM 7300

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Peptone 5 -
Meat extract 3 -
Distilled water - -

Cultivation metadata from 209 strains BacDive

pH 6 - 8 20 strains
pH 4 - 6 14 strains
pH 8 - 10 7 strains
phyla 4 212 strains
nutrition chemoorganotroph 1 strain
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