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REACTIVATION WITH LIQUID MEDIUM 1
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Culture medium recipe
Vol.
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Medium for Sphingomonas melonis DSM 14445
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Main sol. 1a #
ml
| 1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , for solid medium | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 2 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
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Associated strains
| All strains for this medium: | 209 |
| Strains with modifications: | 9 |
Sphingomonas melonis DSM 14445
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Peptone | 5 | - |
| Meat extract | 3 | - |
| Distilled water | - | - |
Cultivation metadata from 209 strains
| pH | 6 - 8 | 20 strains |
| pH | 4 - 6 | 14 strains |
| pH | 8 - 10 | 7 strains |
| phyla | 4 | 212 strains |
| nutrition | chemoorganotroph | 1 strain |
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