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JCM 908 . HALORHODOSPIRA NEUTRIPHILA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

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Medium for Halorhodospira neutriphila DSM 15116

This strain uses the standard medium without modifications.

Main sol. J908 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 0.956 7.025
NH4Cl 0.50 g 0.478 8.936
NaCl 100.00 g 95.602 1635.91
MgCl2 x 6 H2O 2.00 g 1.912 9.405
MgSO4 x 7 H2O 0.50 g 0.478 1.939
CaCl2 x 2 H2O 0.05 g 0.048 0.325
Trace element solution SL-12 1.00 ml - -
NaHCO3
(10%, w/v) 		20.00 ml - -
Na2S x 9 H2O
(10%, w/v) 		7.50 ml - -
Seven vitamins solution 1.00 ml - -
Sodium acetate
(0.5 M) 		4.00 ml - -
Sodium succinate
(0.5 M) 		2.00 ml - -
Yeast extract
(5%) 		10.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0 with 1 M Na2CO3 or 1 M H2SO4. Mix ingredients, except NaHCO3, Na2S x 9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution, and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Separately autoclave acetate solution, succinate solution and yeast extract solution. Filter-sterilize NaHCO3, Na2S x 9H2O. Aseptically and anaerobically add NaHCO3, Na2S x 9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution to the medium.

Trace element solution SL-12 #

1000
Solution occurs in 3 other media (first defined in Medium J496).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 3.0 g 3 -
FeSO4 x 7 H2O 1.1 g 1.1 3.957
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 4 H2O 50.0 mg 0.05 -
ZnCl2 42.0 mg 0.042 0.308
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 18.0 mg 0.018 0.074
H3BO3 300.0 mg 0.3 4.851
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
Distilled water 1000.0 ml - -
1 Autoclave separately.
2 Adjust pH to 6.8.

Seven vitamins solution #

200
Solution occurs in 1 other media (first defined in Medium J699).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 10 mg 0.05 0.037
p-Aminobenzoic acid 10 mg 0.05 0.365
D-(+)-biotin 2 mg 0.01 0.041
Nicotinic acid 20 mg 0.1 0.812
Calcium pantothenate 5 mg 0.025 0.052
Pyridoxine hydrochloride 50 mg 0.25 -
Thiamine-HCl x 2 H2O 10 mg 0.05 -
Distilled water 200 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1
Halorhodospira neutriphila DSM 15116

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 95.602 1635.91
MgCl2 x 6 H2O 1.912 9.405
KH2PO4 0.956 7.025
NH4Cl 0.478 8.936
MgSO4 x 7 H2O 0.478 1.939
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain