Trace element solution SL-12
Internal Solution-ID: S4256
This solution is used in 4 media and was first defined in medium J496.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 3.0 | g | 3 | - | |
| FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| MnCl2 x 4 H2O | 50.0 | mg | 0.05 | - | |
| ZnCl2 | 42.0 | mg | 0.042 | 0.308 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 18.0 | mg | 0.018 | 0.074 | |
| H3BO3 | 300.0 | mg | 0.3 | 4.851 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Autoclave separately. | |||||
| 2 Adjust pH to 6.8. | |||||