J496
496 .
MODIFIED BIEBL AND PFENNIG'S MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J496 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.5 | 3.67 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.699 | |
| MgSO4 x 7 H2O | 1.00 | g | 0.999 | 4.053 | |
| NH4Cl | 0.60 | g | 0.599 | 11.206 | |
|
Yeast extract
(BD-Difco) |
0.30 | g | 0.3 | - | |
| NaCl | 20.00 | g | 19.98 | 341.889 | |
| Sorbitol | 3.00 | g | 2.997 | 16.452 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add the following component after autoclaving: | |||||
| Trace element solution SL-12 | 1.00 | ml | - | - | |
Solution occurs in 3
other media (first defined in Medium J496).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 3.0 | g | 3 | - | |
| FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| MnCl2 x 4 H2O | 50.0 | mg | 0.05 | - | |
| ZnCl2 | 42.0 | mg | 0.042 | 0.308 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 18.0 | mg | 0.018 | 0.074 | |
| H3BO3 | 300.0 | mg | 0.3 | 4.851 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Autoclave separately. | |||||
| 2 Adjust pH to 6.8. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 19.98 | 341.889 |
| Sorbitol | 2.997 | 16.452 |
| MgSO4 x 7 H2O | 0.999 | 4.053 |
| NH4Cl | 0.599 | 11.206 |
| KH2PO4 | 0.5 | 3.67 |
| Show more | ||
