496 .
MODIFIED BIEBL AND PFENNIG'S MEDIUM
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Equipment needed:
Autoclave
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
NH4Cl | 0.60 | g | ||
|
|
Yeast extract
(BD-Difco) |
0.30 | g | ||
|
|
NaCl | 20.00 | g | ||
|
|
Sorbitol | 3.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Add the following component after autoclaving: | |||||
|
|
Trace element solution SL-12 | 1.00 | ml | ||
Trace element solution SL-12 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 3.0 | g | ||
|
|
FeSO4 x 7 H2O | 1.1 | g | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
MnCl2 x 4 H2O | 50.0 | mg | ||
|
|
ZnCl2 | 42.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 18.0 | mg | ||
|
|
H3BO3 | 300.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Autoclave separately. | |||||
| 2 Adjust pH to 6.8. | |||||