Name: BARBOUR-SFOENNER-KELLY (BSK) II MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J173 #

Solution A #

500

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
CMRL 1066 (Sigma, with glutamate)	4.95	g	9.9	-
Bovine serum albumin , Fraction V (Wako)	25.00	g	50	-
HEPES (Sigma)	3.00	g	6	25.177
Trisodium citrate	0.35	g	0.7	2.712
Glucose	2.50	g	5	27.753
Sodium pyruvate (Sigma)	0.40	g	0.8	7.27
N-Acetyl-D-glucosamine (Sigma)	0.20	g	0.4	1.808
NaHCO₃ (Sigma)	1.10	g	2.2	26.188
Yeast extract (BD-Difco, TC)	1.00	g	2	-
Distilled water	500.00	ml	-	-

100

Equipment needed: Autoclave Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Neopeptone (BD-Difco)	2.5	mg	0.025	-
Gelatin	7.0	g	70	-
Distilled water	100.0	ml	-	-
1 Adjust pH to 7.6 with 1 N NaOH and sterilize by filtration through a 0.22 ï¿½m filter (Millipore). Autoclave Solution B, then mix Solutions A and B, and add 40 ml of heat-inactivated (56C, 45 min) sterile rabbit serum (Pel-Freez Biologicals).