Solution B
Internal Solution-ID: S3831
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Neopeptone
(BD-Difco) |
2.5 | mg | 0.025 | - | |
| Gelatin | 7.0 | g | 70 | - | |
| Distilled water | 100.0 | ml | - | - | |
| 1 Adjust pH to 7.6 with 1 N NaOH and sterilize by filtration through a 0.22 �m filter (Millipore). Autoclave Solution B, then mix Solutions A and B, and add 40 ml of heat-inactivated (56C, 45 min) sterile rabbit serum (Pel-Freez Biologicals). | |||||
Media with this solution
| J173 | BARBOUR-SFOENNER-KELLY (BSK) II MEDIUM |