Solution A
Internal Solution-ID: S3830
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
CMRL 1066
(Sigma, with glutamate) |
4.95 | g | 9.9 | - |
|
Bovine serum albumin
, Fraction V
(Wako) |
25.00 | g | 50 | - |
|
HEPES
(Sigma) |
3.00 | g | 6 | 25.177 |
| Trisodium citrate | 0.35 | g | 0.7 | 2.712 |
| Glucose | 2.50 | g | 5 | 27.753 |
|
Sodium pyruvate
(Sigma) |
0.40 | g | 0.8 | 7.27 |
|
N-Acetyl-D-glucosamine
(Sigma) |
0.20 | g | 0.4 | 1.808 |
|
NaHCO3
(Sigma) |
1.10 | g | 2.2 | 26.188 |
|
Yeast extract
(BD-Difco, TC) |
1.00 | g | 2 | - |
| Distilled water | 500.00 | ml | - | - |
Media with this solution
| J173 | BARBOUR-SFOENNER-KELLY (BSK) II MEDIUM |